CRISPR-Cas9: Current Status and Applications - Literature Review
CRISPR-Cas9: Current Status and Applications - Literature Review By: Keerthana Bellary Abstract Genome editing technologies have revolutionized biotechnology by enabling precise modifications to DNA sequences. Among these, CRISPR-Cas9 stands out for its efficiency and versatility in manipulating genetic material. Originally adapted from bacterial defense mechanisms, CRISPR-Cas9 utilizes a guide RNA (gRNA) to direct the Cas9 enzyme to specific DNA sequences, facilitating targeted modifications such as gene knockout, insertion, or correction. This review explores the current landscape and future prospects of CRISPR-Cas9, focusing on its applications in medicine, agriculture, and biotechnology. Ergo, CRISPR-Cas9 has transformed genetic research and application domains, offering unprecedented opportunities for genomic interventions. Future efforts are directed towards enhancing specificity, broadening applicability across diverse organisms, and addressing regulatory and ethi...